Microbial Dynamics seminar today at 3pm in the Suddath Room (IBB 1128)

"Iterative Subtractive Binning of Freshwater Chronoseries Metagenomes Recovers Nearly Complete Genomes of over Four Hundred Novel Species"

The catalogue of available genomes from uncultivated freshwater microbial populations remains limited despite recent advances in sequencing and bioinformatics methods, and most genome recovery attempts in freshwater ecosystems have only targeted few specific taxa. We present a novel genome recovery pipeline, which incorporates iterative subtractive binning and apply it to a time series of metagenomic datasets from seven connected locations along the Chattahoochee River (Southeastern USA). Our set of Metagenome-Assembled Genomes (MAGs) represents over four hundred genomospecies yet to be named, which substantially increase the number of high-quality MAGs from freshwater lakes and represent, on average, ~50% of the total microbial community sampled. To evaluate the prevalence of these species in the chronoseries, we introduce novel approaches to estimate relative abundance and a habitat-preference score that control for uneven quality of the genomes and sample representation. Using these metrics, we demonstrate a high degree of habitat-specialization and endemicity for most genomospecies observed in the Chattahoochee lacustrine ecosystem, as well as wider species ecological ranges associated with smaller genomes and higher coding densities, indicating an overall advantage of smaller, more compact genomes for cosmopolitan distributions.

Event Details


  • Monday, September 9, 2019
    3:00 pm - 4:00 pm
Location: IBB 1128

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